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Revised protocol. Optimized transformation, overexpression and purification of S100A10

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posted on 2019-09-24, 15:08 authored by Xiaolin YangXiaolin Yang, Marie-France Lebel-Beaucage, Samuel Tremblay, Line Cantin, Gary S. Shaw, Elodie Boisselier
PROTOCOL FOR: Optimized transformation, overexpression and purification of S100A10


The authors are indebted to the Natural Sciences and Engineering Research Council of Canada (NSERC), the Eye Disease Foundation, the CHU de Québec Foundation for their financial support. EB is a research scholar from the Fonds de recherche du Québec - Santé (FRQS) in partnership with the Antoine Turmel Foundation. XY is a PhD student that received a scholarship from The Quebec Network for Research on Protein Function, Engineering, and Applications (PROTEO). GSS acknowledges funding from the Canadian Institutes of Health Research (MOP-93520). The authors would like to thank Enago (www.enago.com) for the English language review. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject