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Supplementary figures: Cytotoxic T lymphocytes targeting a conserved SARS-CoV-2 spike epitope are efficient serial killers

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posted on 2022-03-17, 13:51 authored by Daniel Meyer, Mohsen Fathi, Lindsey Charley, Laurence Cooper, Navin Varadarajan

Supplementary Figure 1: (A) Multiple sequence alignment of the conserved epitope (DD9) on the spike protein of SARS-CoV-2 variants; YP_009724390 (Wuhan), B.1.617.2 (Delta), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.1.529 (Omicron). All sequences were downloaded from NCBI and aligned by Clustal Omega. (B) 3D structure of SARS-CoV-2 spike protein (PDB ID 6VYB) highlighted with DD9 peptide in red (Constructed in Jmol: an open-source Java viewer for chemical structures in 3D. http://www.jmol.org/).

Supplementary Figure 2: (A) The distribution of T cells (effector) and target cells (target) in the nanowell array. (B) The Kaplan Meier survival curve of target cells in absence (0E:1T) and presence of one T cell (1E:1T).

Supplementary Figure 3: The percentage of target cell apoptosis in different E:T ratios in a bulk assay for 48 hours (Cytation). All target cells were pre-incubated with peptide for 60 minutes before co-culturing.