Characterization of the impact of MYBBP1A gene and rs3809849 on asparaginase sensitivity and cellular functions: Supplementary material.docx
Supplemental Figure S1. Production of Cas9 expressing, & MYBBP1A gene knock-out PANC1 cell lines.
Supplemental Figure S2. Production of MYBBP1A gene knock-out in Cas9 expressing NALM6 cell line.
Supplemental Figure S3. INDEL spectrum and TIDE analysis of the efficacy of the knock-in experiments performed to produce the a) PANC1-Cas9-MYBBP1A-Gln8-His8 and b) NALM6-Cas9-mybbp1a-rs38098449-MUT cell lines.
Supplemental Figure S4. Illustration of the general construct of the pLenti plasmid vectors used for the transfection of the PANC1 cells.
Supplemental Figure S5. In-vitro sensitivity to asparaginase and vincristine in relation to MYBBP1A gene knock-out 96 hours post incubation with a) asparaginase (ASNase) or b) vincristine (VCR).
Supplemental Figure S6. Steps used in the validation and clonal selection of MYBBP1A overexpressing vectors in PANC1 cell lines.
Supplemental Figure S7. Association of MYBBP1A gene expression levels with survival probability in different types of cancer.
Supplemental Figure S8. Relative expression of p53 in response to MYBBP1A gene deletion and asparaginase exposure.
Supplemental Figure S9. Association between rs3809849 and the expression of GGT6 gene based on the genotype.
Supplemental Table S1. List of primers used for qPCR
Details of methods.