Supplementary Protocol for. Optimization of a peptide extraction and LC–MS protocol for quantitative analysis of antimicrobial peptides: supplementary material

Chen, Wen; Hwang, Yoon Y; Gleaton, Jeremy W; Titus, James K; Hamlin, Nicholas J

doi:10.25402/FSOA.7868420.v2

supplementary_protocol.docx (33.16 kB)

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Supplementary Protocol for. Optimization of a peptide extraction and LC–MS protocol for quantitative analysis of antimicrobial peptides: supplementary material

journal contribution

posted on 2019-03-20, 14:06 authored by Wen Chen, Yoon Y Hwang, Jeremy W Gleaton, James K Titus, Nicholas J Hamlin

The authors optimized a peptide extraction and LC–MS protocol for identification and quantification of antimicrobial peptides (AMPs) in biological samples. This document describes that protocol step-by-step

Funding

This work was supported by the Naval Medical Research Center's Advanced Medical Development Program, Work Unit Numbers G1722 and G1723

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Keywords

antimicrobial peptide identification and quantification liquid chromatography mass spectrometry peptide extraction Analytical Biochemistry Microbiology not elsewhere classified Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)

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CC BY 4.0

Supplementary Protocol for. Optimization of a peptide extraction and LC–MS protocol for quantitative analysis of antimicrobial peptides: supplementary material

Funding

This work was supported by the Naval Medical Research Center's Advanced Medical Development Program, Work Unit Numbers G1722 and G1723

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