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Reducing Mitomycin-C-induced ROS Levels in Mouse Feeder Cells Improves Induced Pluripotent Stem Cell Colony Growth

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posted on 2020-01-15, 11:31 authored by Fatima Mariz Almenario, Ranelle Janine L. Asi, Sonia D. Jacinto, Ahmad Reza F. Mazahery

Figure S1. Phase-contrast images of SL10 feeder cells treated with mitomycin-C (MMC) at varying exposure times.

Figure S2. Revival of iPSC using Primate embryonic stem cell media (PreESM) without bFGF.

Figure S3. Withdrawal of bFGF in active culture of induced pluripotent stem cells (iPSCs) results in premature differentiation
Figure S4. Feeder-free culture of induced pluripotent stem cells (iPSCs) using matrigel and classic Primate embryonic stem cell media (PrESM) and StemMacs (SM).

Funding

This study was funded by the Natural Science Research Institute (Grant no. BIO-18-1-05), and the Office of the Vice Chancellor for Research and Development, University of the Philippines Diliman (Grant no. 181818PhDIA).

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    BioTechniquesBioTechniques

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    Keywords

    Feeder-dependent cultureMitomycin-C treatmentMouse embryonic fibroblastsInduced pluripotent stem cellsReactive oxygen speciesStem CellsProteins and Peptides

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