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Expression of mouse small interfering RNAs in lettuce using artificial microRNA technology

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posted on 2020-01-15, 11:28 authored by Tayebeh Kakeshpour, Tej Man Tamang, Woojun D Park, Murli Manohar, Jian YangJian Yang, Kendal D. Hirschi, Sunghun Park

Figure S1 - PCR scheme to produce artificial microRNA constructs

Figure S2 - Absolute quantification of transgene copy number in C3siRNA- and CF7siRNA-expressing lettuces using quantitative real-time PCR
Figure S3 - Quantitative real-time PCR analysis of mature C3 and CF7 artificial microRNAs (amiRNAs) in C3siRNA- and CF7siRNA-expressing lettuces using stem-loop primer.

Funding

This project has been funded by Agriculture and Food Research Initiative competitive grant no. 2016-67017-24712 from the USDA National Institute of Food and Agriculture (SHP), National Science Foundation grant no. IOS-1741090 (SHP), and U.S. Department of Agriculture, Agricultural Research Service, under Cooperative agreement number 58-3092-5-001 (KDH)

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    BioTechniquesBioTechniques

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    Keywords

    amiRNA technologylettucemousePCRtherapeutic dietary siRNAGenomicsGene Expression (incl. Microarray and other genome-wide approaches)

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