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Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling: Supplementary Tables

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posted on 26.02.2020 by Hui Zhang, Xiangdan Yu, Zhe Zhang, Zhenhuan Liu, Cong Tang, Shiyan Liu, Kun Zhao, Yong Sun, Xiang Li, Zijing Xu, Hong Zhang, Keqing Shi, Yan Fu

Supplementary Table 1. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

Primer sequences. The inner primer pairs were used to amplify the V3/V4 region of the 16S rRNA gene. The outer primers with spacer sequences and SP sequences at the 5´region of primer were used to construct SNAP-TE libraries compatible for Illumina MiSeq/HiSeq platform.

Supplementary Table 2. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

Index sequences used for next-generation sequencing library construction.

Supplementary Table 3. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

A total of 14 flexible sample/assay combination modes that can set on multi-sample nanodispenser system. Replicates can be easily done using these settings.

Supplementary Table 4. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

All parameters tested for SNAP-TE experiments. Two replicates of 24 nanowells on a chip were used for each test of PCR system conditions. Data from individual next-generation sequencing libraries from each nanowell were pooled by samples for analyses.

Supplementary Table 5. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

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