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Supplementary Video 2. Fast, versatile and quantitative annotation of complex images

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posted on 04.06.2019 by Kathleen Bates, Shen Jiang, Shivesh Chaudhary, Emily Jackson-Holmes, Melinda L Jue, Erin McCaskey, Daniel I Goldman, Hang Lu

Supplementary Video 2. Fast, versatile and quantitative annotation of complex images

Representative example reconstruction of worm behavior. Segmented images from raw data are on left, with purely algorithmic reconstruction in center, and consensus annotation-based reconstruction on right (only for complex or ambiguous frames). Although algorithmic reconstruction is visually more accurate in many frames, annotation-based reconstruction produces accurate results even in frames that are inaccurately segmented or in cases where the algorithm performs poorly. Movie is at half speed from original 30 fps.

Funding

KB received funding from the National Institute of General Medical Sciences (US NIGMS), award number F31 GM123662. HL received funding from the National Institute of General Medical Sciences (US NIGMS), award number R01GM088333 and the US National Science Foundation STC EBICS 0939511. Some C. elegans strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440)

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