Figure S2.pdf (3.3 MB)

Supplementary Figure 2. Rapid Identification of Genome Edited Mesenchymal Stem Cell Colonies via Cas9

Download (3.3 MB)
journal contribution
posted on 29.03.2019 by Wei Jiang, Wei Lian, Jieting Chen, Wenlei Li, Jieyong Huang, Baoyu Lai, Lingyun Li, Zhong Huang, Jianyong Xu

Supplementary Figure 2. Target inserting the IL-37 into AASV1 site. (A) Donor and gRNA structures. (B) PCR characterization of genome edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR product size. (C) PCR characterization of genome edited MSC colonies by amplifying the right arm. Arrow shows the predicted PCR product size. (D) mRNA level of IL-37 among the colony 8 (C8) without donor insertion, colony 20 (C20) with correct insertion, and the wild type of MSCs (NC). M: DNA ladder; arrows showed the DNA size with 750bp, 1.0kb or 2kb.


Funding

Natural Science Foundation of Shenzhen JCYJ20170302152735071

Natural Science Foundation of Shenzhen JCYJ20170818093720089

Natural Science Foundation of SZU 2017083

Natural Science Foundation of SZU 2017082

Medical Foundation of Guangdong A2018308

History

Exports