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Ultrasensitive digital immunoassays for SOD1 conformation in amyotrophic lateral sclerosis: supplementary figures

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posted on 2023-08-31, 11:39 authored by Lisa Morichon, Christophe Hirtz, Laurent Tiers, Alexandre Mezghrani, Cedric Raoul, Florence Esselin, Elisa De La Cruz, Jean-Pierre Julien, William Camu, Sylvain Lehmann

Aim: The aim of this study was to detect misfolded Cu/Zn SOD1 as a potential biomarker for amyotrophic

lateral sclerosis (ALS). Materials & methods: Two ultrasensitive immunodetection assays were developed

for the quantification of total and misfolded SOD1. Results: The detection of total and misfolded

SOD1 was possible in human serum and cerebrospinal fluid. Total SOD1 was increased in cerebrospinal

fluid from ALS patients. Misfolded SOD1 had low and variable expression in both control and ALS patient

samples. Conclusion: These assays hold promise for improving our understanding of ALS and its detection,

and could lead to more effective treatment options in the future. Further studies in larger cohorts are now



This work was supported by the association for the research on ALS (ARSLA) (project no. misSOD1).