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Target sequence heterogeneity causes the ‘hook effect’ in fluorescent dye based quantitative PCR: Supplementary Figure 1

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posted on 05.06.2020 by Kristina Warton, Yue Xu, Caroline Elizabeth Ford
Supplementary Figure 1: Data from ­gure 1A with ‑uorescence shown on log scale. Fluorescence acquisition below the heteroduplex melt temperature (72°C, green trace) shows a plateau after ampli­cation; ‑uorescence acquisition above the heteroduplex melt temperature shows the ‘hook eect’ (77°C, purple trace).