Supplementary Figure 1.pdf (72.75 kB)
Download fileTarget sequence heterogeneity causes the ‘hook effect’ in fluorescent dye based quantitative PCR: Supplementary Figure 1
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posted on 2020-06-05, 10:56 authored by Kristina Warton, Yue Xu, Caroline Elizabeth FordSupplementary Figure 1: Data from
gure 1A with ‑uorescence shown on log scale. Fluorescence acquisition below
the heteroduplex melt temperature (72°C, green trace) shows a plateau after
amplication; ‑uorescence acquisition above the heteroduplex melt temperature
shows the ‘hook eect’ (77°C, purple trace).