Supplementary Figure 1.pdf (72.75 kB)
Target sequence heterogeneity causes the ‘hook effect’ in fluorescent dye based quantitative PCR: Supplementary Figure 1
figureposted on 2020-06-05, 10:56 authored by Kristina Warton, Yue Xu, Caroline Elizabeth Ford
Supplementary Figure 1: Data from gure 1A with ‑uorescence shown on log scale. Fluorescence acquisition below the heteroduplex melt temperature (72°C, green trace) shows a plateau after amplication; ‑uorescence acquisition above the heteroduplex melt temperature shows the ‘hook eect’ (77°C, purple trace).