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Supplementary information - HA/PEI-coated Acridine Orange-loaded gold-core silica shell nanorods for cancer-targeted photothermal and chemotherapy

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posted on 02.12.2021, 11:32 by Carolina F. Rodrigues, Natanael Fernandes, Duarte de Melo-Diogo, Paula Ferreira, Ilídio J. Correia, André F. Moreira

Supplementary Methods

Figure S1: Schematic synthesis of PEI-TESPIC polymer.

Figure S2: FTIR spectrum of PEI-TESPIC polymer.

Figure S3 – Schematics of AuMSS functionalization’ approaches with the PEI and HA polymers.

Figure S4 - Photothermal conversion efficiency of AuMSS and AuMSS/PEI/HA nanorods. Data are presented as mean±s.d., n=3.

Figure S5 - Dose-response curve of AO in HeLa Cells.

Figure S6 - Optical images of the blood supernatants and RBCs pellet recovered after AuMSS, AuMSS/PEI and AuMSS/PEI/HA nanorods incubation for 24h. PBS was used as negative control (K-) and Triton-X 100 was used as a positive control for red blood cells’ lysis (K+).

Figure S7 - Analysis of the cytotoxic activity of AuMSS and AuMSS/PEI/HA (100 µg/mL), with or without AO encapsulation or NIR laser irradiation (5min). Negative control (K-): cells without nanoparticles incubation; NIR control (K NIR): cells without nanoparticles incubation and irradiated with NIR laser (808 nm, 1.7 W/cm2, 5 min). Data are presented as mean ± standard deviation, n=5.


This work was financed by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB / 00709/2020. The funding from CENTRO-01-0145-FEDER-028989 and POCI-01-0145-FEDER-031462 are also acknowledged. Carolina F. Rodrigues acknowledges for her Ph.D. fellowship from FCT (SFRH/BD/144680/2019). Duarte de Melo-Diogo acknowledges CENTRO-01-0145-FEDER-028989 for the funding given in the form of a research contract.