Supplementary information - HA/PEI-coated Acridine Orange-loaded gold-core silica shell nanorods for cancer-targeted photothermal and chemotherapy
Figure S1: Schematic synthesis of PEI-TESPIC polymer.
Figure S2: FTIR spectrum of PEI-TESPIC polymer.
Figure S3 – Schematics of AuMSS functionalization’ approaches with the PEI and HA polymers.
Figure S4 - Photothermal conversion efficiency of AuMSS and AuMSS/PEI/HA nanorods. Data are presented as mean±s.d., n=3.
Figure S5 - Dose-response curve of AO in HeLa Cells.
Figure S6 - Optical images of the blood supernatants and RBCs pellet recovered after AuMSS, AuMSS/PEI and AuMSS/PEI/HA nanorods incubation for 24h. PBS was used as negative control (K-) and Triton-X 100 was used as a positive control for red blood cells’ lysis (K+).
Figure S7 - Analysis of the cytotoxic activity of AuMSS and AuMSS/PEI/HA (100 µg/mL), with or without AO encapsulation or NIR laser irradiation (5min). Negative control (K-): cells without nanoparticles incubation; NIR control (K NIR): cells without nanoparticles incubation and irradiated with NIR laser (808 nm, 1.7 W/cm2, 5 min). Data are presented as mean ± standard deviation, n=5.