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Supplementary figures: Development of multiplexed RT-LAMP for detection of SARS-CoV-2 and influenza viral RNA

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posted on 04.02.2021, 09:43 by Nathan A. Tanner, Yinhua Zhang

Supplementary Figure 1. Detection of Influenza A RNA and an internal control in the presence multiple primer sets. Reactions were performed with 24 repeats each containing 1 μl of Flu A RNA diluted to 1/10000 or 8 repeats as NTC using both IAV and ACTB primer sets or with additional primer sets. The results were organized the same way as in Figure 2. A. Dual (IAV + ACTB) DARQ LAMP amplification. B. Dual (IAV + ACTB) DARQ LAMP amplification in the presence of a unrelated primer set E1 and its QFIP:Fd duplex. C. Dual (IAV + ACTB) DARQ LAMP in the presence of 2 unrelated primer sets E1 and IBV and their QFIP:Fd duplexes. D. ACTB detection in C. E. Summary of detection: total number of positives, amplification speed (Cq) and correlation of results by real-time curve and end point scanning.

Supplementary Figure 2. Detection of Influenza B RNA and an internal control in the presence multiple primer sets. Reactions were performed with 24 repeats each containing ~21 copies of Influenza B RNA or 8 repeats as NTC using IBV and ACTB primer sets or with additional primer sets. The results were organized the same way as in Figure 2. A. Dual (IBV + ACTB) DARQ LAMP amplification. B. Dual (IBV + ACTB) DARQ LAMP amplification in the presence of a unrelated primer set E1 and its QFIP:Fd duplex. C. Dual (IBV + ACTB) DARQ LAMP in the presence of 2 unrelated primer sets E1 and IAV and their QFIP:Fd duplexes. D. ACTB detection in C. E. Summary of detection: total number of positives, amplification speed (Cq) and correlation of results by real-time curve and endpoint scanning.

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New England Biolabs

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