Supplementary figure 1 – Applicability of LN2
method to break ddPCR emulsion. ddPCR reactions were prepared with ddPCR™
Supermix for Probes (no dUTP) and Droplet Generation Oil for Probes (three
tubes on the left) or ddPCR™ Supermix for EvaGreen® and Droplet Generation Oil
for EvaGreen® (three tubes on the right). The tubes (A) and (D) show the
emulsion before using LN2 method, and tubes (B), (C), (E) and (F) shows the oil
and water layers after breaking the emulsion.