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Supplementary figure: A cryostat-based frozen section method to increase the yield of EV extracted from different tissues

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posted on 2022-08-15, 13:56 authored by Shuangzhu Yang, Weiwei Qin, Xiangji Li, Qingdong Guo, Yuan Wang, Jing Chen, Hongmei Li, Shutian Zhang, Li Min

      

Figure s1.

(a) The protein concentration of tissue dissociation reagent before and after SEC; (b) The nanoparticle counting of EV sample and tissue dissociation reagent after SEC; (c) The nanoparticle counting of PBS solution. 

Funding

This work was financially supported by the National Natural Science Foundation of China (82073390, 81702314), Beijing Science and Technology Nova Program (Z191100001119128), Beijing Municipal Science and Technology Project (Z191100006619081), The Digestive Medical Coordinated Development Center of Beijing Municipal Administration of Hospitals (XXZ02, XXZ01).

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    BioTechniques

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    Keywords

    extracellular vesiclestissuesultracentrifugationultrafiltrationsize-exclusion chromatography

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