Supplementary figure 3. Conducting polymer-based multilayer films for instructive biomaterial coatings
Supplementary Figure S3
HUMAN MESENCHYMAL STEM CELL ADHESION STUDIES
HMSCs were supplied by Lonza (Walkersville, MD). Samples were prepared as described above. After sterilization, the samples were incubated for 30 minutes in 24 well plates containing HMSC growth medium that was composed of: high glucose Dulbecco’s Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL); antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng mL-1 basic fibroblast growth factor. Medium was aspirated and replaced prior to HMSC seeding. Cell viability before starting the experiment was determined by the Trypan Blue exclusion method, and the measured viability exceeded 95 % in all cases. HMSCs were seeded at 5,000 cells cm-2, and incubated at 37 °C, 95 % humidity, and a CO2 content of 5 %. Samples were fixed and stained as decribed above (n = 3).
- HMSCs on multilayer films after 3 days in culture. A) PEDOT-PSS-based multilayer films. B) PEDOT-S-based multilayer films. DAPI-stained nuclei are blue and Alexa Fluor® 488-stained actin is green. Scale bars represent 150 µm.