Supplementary figure 2: Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR
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<tr>
<td>
<p>Supplemental figure 1A and B: Agarose gel
after PCR.</p>
<p>A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.</p>
<p>Supplemental figure 2A and B: Agarose gel
after purification of PCR products.</p>
<p>A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.</p>
<p>Supplementary material 1: Protocol for Agarose
gel electrophoresis to assess PCR product yield – Comparison with
spectrophotometry, fluorometry and qPCR. </p>
<p>Supplementary table
1: PCR reaction composition</p>
<p>Supplementary table
2: PCR reaction parameters</p>
<p>Supplementary table
3: Primer sequences</p>
<p>Supplementary table 4: Peak area acquired
with ImageJ from agarose gel after PCR</p>
<p>Supplementary table 5: Peak area acquired
with ImageJ from agarose gel after PCR product purification</p>
<p>Supplemental material 3 containing: Supplementary
table 6: Results of spectrophotometry</p>
<p>Supplementary table 7: Results of
fluorometry</p>
<p>Supplementary table 8: Results of qPCR</p>
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</tr>
</table>
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