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Supplementary figure 1: Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

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posted on 21.03.2022, 10:44 by Patrick Wittmeier, Susanne HummelSusanne Hummel

Supplemental figure 1A and B: Agarose gel after PCR.

A: Original image of agarose gel. B: Image of agarose gel with virtually subtracted background.

Supplemental figure 2A and B: Agarose gel after purification of PCR products.

A: Original image of agarose gel. B: Image of agarose gel with virtually subtracted background.

Supplementary material 1: Protocol for Agarose gel electrophoresis to assess PCR product yield – Comparison with spectrophotometry, fluorometry and qPCR.

Supplementary table 1: PCR reaction composition

Supplementary table 2: PCR reaction parameters

Supplementary table 3: Primer sequences

Supplementary table 4: Peak area acquired with ImageJ from agarose gel after PCR

Supplementary table 5: Peak area acquired with ImageJ from agarose gel after PCR product purification

Supplemental material 3 containing: Supplementary table 6: Results of spectrophotometry

Supplementary table 7: Results of fluorometry

Supplementary table 8: Results of qPCR

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