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Supplementary data: Methods to evaluate the impact of SARS-CoV-2 nucleocapsid mutations on antigen detection by rapid diagnostic tests

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posted on 2022-08-25, 07:58 authored by Bryan C Tieman, Stephen Kovacs, Mary A. Rodgers, Aurash Mohaimani, Svetoslava Gregory, David Christensen, Jeffrey A. Moore, Lici A. Schurig-Briccio, James R. Hartnett, Alak Kanti Kar, Aaron Leeman, Angela C. Palmer, Lauren Rogers, Brian Dragoo, Steven Muszynski, Deborah Noblesmith, Samantha Gardner, Abigail Snipe-Bushey, Anna Kill, Xinxin Luo, Sneha Cherukuri, Tracey Rae, Christopher C Marohnic, Gavin A. Cloherty, A. Scott Muerhoff, Philip M Hemken

      

Supplemental   Table S1. SARS-CoV-2 Variants Included in the Analysis

Supplemental   Table S2. Anti-nucleocapsid   Antibodies Used in Each Assay

Supplemental Figure S1. Representative immunoblots   of all recombinant SARS-CoV-2 nucleocapsid antigens (rAg; see Table 1) with   anti-nucleocapsid antibodies used   in the Panbio assay (Ab1, Ab2) and BinaxNOW assay (Ab3, Ab4).

Supplemental   Figure S2. Normalized   Sensorgrams of Ab1 Binding Captured SARS-CoV-2 Nucleocapsids. 

Supplemental   Figure S3. Normalized   Sensorgrams of Ab2 Binding Captured SARS-CoV-2 Nucleocapsids

Funding

This work was supported by Abbott Laboratories. All authors are employees of Abbott Laboratories. Medical writing and editorial support was provided by Stacey Tobin, PhD, funded by Abbott Laboratories.

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