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Supplementary data: Methods to evaluate the impact of SARS-CoV-2 nucleocapsid mutations on antigen detection by rapid diagnostic tests

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posted on 2022-08-25, 07:58 authored by Bryan C Tieman, Stephen Kovacs, Mary A. Rodgers, Aurash Mohaimani, Svetoslava Gregory, David Christensen, Jeffrey A. Moore, Lici A. Schurig-Briccio, James R. Hartnett, Alak Kanti Kar, Aaron Leeman, Angela C. Palmer, Lauren Rogers, Brian Dragoo, Steven Muszynski, Deborah Noblesmith, Samantha Gardner, Abigail Snipe-Bushey, Anna Kill, Xinxin Luo, Sneha Cherukuri, Tracey Rae, Christopher C Marohnic, Gavin A. Cloherty, A. Scott Muerhoff, Philip M Hemken


Supplemental   Table S1. SARS-CoV-2 Variants Included in the Analysis

Supplemental   Table S2. Anti-nucleocapsid   Antibodies Used in Each Assay

Supplemental Figure S1. Representative immunoblots   of all recombinant SARS-CoV-2 nucleocapsid antigens (rAg; see Table 1) with   anti-nucleocapsid antibodies used   in the Panbio assay (Ab1, Ab2) and BinaxNOW assay (Ab3, Ab4).

Supplemental   Figure S2. Normalized   Sensorgrams of Ab1 Binding Captured SARS-CoV-2 Nucleocapsids. 

Supplemental   Figure S3. Normalized   Sensorgrams of Ab2 Binding Captured SARS-CoV-2 Nucleocapsids


This work was supported by Abbott Laboratories. All authors are employees of Abbott Laboratories. Medical writing and editorial support was provided by Stacey Tobin, PhD, funded by Abbott Laboratories.