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Supplementary data 1: A single-wavelength, flow cytometric approach using roGFP probes for measuring redox stress in live cells

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posted on 18.05.2021, 08:45 by Joseph Schober, Elizabeth Denn

Figure S1. Localization of cyto-roGFP and matrix-roGFP in CHO and MDA-MB-231 cells. CHO (A) and MDA-MB-231 (B) cells expressing cyto-roGFP or matrix-roGFP were trypsinized and plated onto Fn-coated coverslips, fixed, and then stained for visualization of the nucleus and actin. Representative images are shown. Scale bar = 20 m

Figure S2. Co-localization of matrix-roGFP with Mitotracker RED CMXRos staining in CHO and MDA-MB-231 cells. CHO (A) and MDA-MB-231 (B) cells expressing matrix-roGFP were trypsinized and plated onto Fn-coated coverslips, fixed, and then stained for visualization of mitochondria. Representative images are shown. Scale bar = 20 m

Funding

This work was supported by Research Grants for Graduate Students (RGGS) from Southern Illinois University Edwardsville (SIUE).

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