Table S1. Oligonucleotide sequences used in this study
Figure S1. Library yield depending on the number and position of phosphorothioate bonds in the
adapter oligonucleotides. The used adapters contained different numbers of phosphorothioate
bonds at different positions within the oligonucleotide. Oligonucleotide designations: BC, barcode number of the Ion Xpress index sequence; pt, adapter oligonucleotide containing given number of
phosphorothioate bonds; bc, phosphorothioate bonds located upstream of the barcode within the
key sequence. For further details and the oligonucleotide sequences, see Table 1 in the main
document. All libraries were prepared of the same batch of fragmented E. coli DNA in two independent experiments; vertical lines indicate the standard deviation calculated from three dilutions used in qPCR quantification. The horizontal line indicates the mean concentration of all libraries shown in the plot, the dashed lines indicate the mean ± 1 standard deviation
analysis of libraries prepared using standard-and Y-adapters. Melting curves were
recorded after qPCR quantification for 0ng, 2 ng, 10 ng, and 50 ng DNA input.
In comparison with the reference curves, the presence of adapter dimers is
clearly visible. Reference was assembled of single peaks for non-template
control (primer dimer; yellow), library without input (adapter dimer; red) and
library quantification standard (153 bp; black).