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Download fileSupplementary Material : Gel purification of genomic DNA for NGS applications
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posted on 2022-08-11, 07:41 authored by Supanut Utthiya, Passorn Wonnapinij, Pondpan Napaumpaiporn, Chokchai Kittiwongwattana, Jenjira Sakulkoo, Anongpat Suttangkakul, Supachai VuttipongchaikijSupachai Vuttipongchaikij
Supplementary Material 1. Total nucleic acid and gel-purified gDNA of dictyostelids obtained from gel electrophoresis using non-autoclaved running buffer. (M) indicates a DNA ladder.
Supplementary Material 2. EcoRI digestion of gDNA obtained from gel purification. gDNA (200 ng per lane) was incubated in EcoRI digestion reactions at 37°C for 2 hr. Controls were performed by replacing the EcoRI enzyme with ultrapure water. (M) indicates a DNA ladder.
Supplementary Material 3. NGS read quality from gel-purified gDNA of dictyostelid strains. The qualities of paired-end reads of 150 bp from each end were analyzed by FastQC. Total reads for each strains are presented.
