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Supplementary Material : Gel purification of genomic DNA for NGS applications

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posted on 2022-08-11, 07:41 authored by Supanut Utthiya, Passorn Wonnapinij, Pondpan Napaumpaiporn, Chokchai Kittiwongwattana, Jenjira Sakulkoo, Anongpat Suttangkakul, Supachai VuttipongchaikijSupachai Vuttipongchaikij

      

Supplementary   Material 1. Total nucleic acid and gel-purified gDNA of dictyostelids   obtained from gel electrophoresis using non-autoclaved running buffer. (M) indicates a DNA   ladder.   

Supplementary   Material 2. EcoRI digestion of gDNA obtained from gel purification. gDNA (200   ng per lane) was incubated in EcoRI digestion reactions at 37°C   for 2 hr. Controls were performed by replacing the EcoRI enzyme with   ultrapure water. (M) indicates a DNA ladder.  

Supplementary Material 3. NGS read quality from gel-purified gDNA of dictyostelid strains. The qualities of paired-end reads of 150 bp from each end were analyzed by FastQC. Total reads for each strains are presented. 

Funding

This work was supported by Kasetsart University Research and Development Institute (KURDI) (FF(KU)13.64). SU was supported by studentship from Faculty of Science, Kasetsart University. SV was supported by National Research Council of Thailand (NRCT5-RSA63002-02) and The Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021.

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