Supplementary Figures – Solid lipid nanoparticles loaded with curcumin: development and in vitro toxicity against CT26 cells

Figure S1. Polydispersity index (PDI, y-axis on the left) and hydrodynamic diameter (y-axis on the right) variations of solid lipid nanoparticles with curcumin and blank nanoparticles (SLN-C and SLN, respectively) during 300 days.

Figure S2. DLS measurements for the different solid lipid nanoparticles (SLN). Samples at pH 1.2: (a) Blank SLN; (c) Blank SLN and mucin; (b) SLN with curcumin; and (d) SLN with curcumin and mucin. Samples at pH 6.8: (e) Blank SLN; (g) Blank SLN and mucin; (f) SLN with curcumin; and (h) SLN with curcumin and mucin.

Figure S3. Confocal laser scanning microscopy images of the internalization of free or nanostructured curcumin (SLN-C) in CT26 cells after 4 or 24 h of exposure. To evaluate internalization, a concentration of 10 μg/mL curcumin was used. A) and B) control cells in 4 and 24 h, respectively, in transmission mode. C) and D) Superposition of transmission and fluorescence modes for cell exposed to free curcumin after 4 and 24 h, respectively; E) and F) Superposition of transmission and fluorescence modes for cell exposed to SLN-C after 4 and 24 h, respectively.

Figure S4. Morphology alterations regarding the granularity of CT26 cells. Light microscopy images of (A) control and (B) exposed cells to SLN-C for 24 h. Red arrows indicate the granules in the cells.