posted on 2021-09-14, 09:10authored byMenachem Ailenberg, Andras Kapus, Ori D Rotstein
Efficiency of D-B primers in detecting SARS-COV-2 virus in nasopharyngeal
swabs. cDNA of reverse-transcribed RNA extracted from nasopharyngeal swabs of
patient S1 underwent 3 decimal dilutions and subjected to qPCR with SYBR
green kit+ UDG and D-B primer mix #8.(A) amplification plot and
insert efficiency plot of Ct vs log dilution. Slope= -3.2942
representing 101.27% amplification efficiency and R2= 0.9934. (B)
melt curve of the amplicons depicted in panel A. Ct dilution values: 100-
25.55, 10-1- 28.38, 10-2- 32.14, NTC- 39.48. qPCRwas performed under
standard conditions.
Funding
This study was supported by a grant from the St. Michael’s Hospital Foundation (OR, AK) and a grant from CIHR # 400642 (OR).