Supplementary Figure S6: Improved SARS-COV-2 PCR detection and genotyping with double-bubble primers
Efficiency of D-B primers in detecting SARS-COV-2 virus in nasopharyngeal swabs. cDNA of reverse-transcribed RNA extracted from nasopharyngeal swabs of patient S1 underwent 3 decimal dilutions and subjected to qPCR with SYBR green kit+ UDG and D-B primer mix #8. (A) amplification plot and insert efficiency plot of Ct vs log dilution. Slope= -3.2942 representing 101.27% amplification efficiency and R2= 0.9934. (B) melt curve of the amplicons depicted in panel A. Ct dilution values: 100- 25.55, 10-1- 28.38, 10-2- 32.14, NTC- 39.48. qPCR was performed under standard conditions.