Supplementary Figure S3: Improved SARS-COV-2 PCR detection and genotyping with double-bubble primers
Melt curve plot of double dilutions of PCR amplicons of SARS-COV-2 viral RNA extracted from nasopharyngeal swabs of patient S1. RNA was subjected to one-tube RT-PCR and amplicons were subjected to double-dilutions (#1 the most concentrated, #9 the most diluted and #10 no amplicon). SYBR green and ROX dyes were added and melt curve was performed. Data show derivative reporter. These findings are consistent with the no-shift finding shown in Figure 1E.