posted on 2021-09-14, 09:10authored byMenachem Ailenberg, Andras Kapus, Ori D Rotstein
Melt curve
plot of double dilutions of PCR amplicons of SARS-COV-2 viral RNA extracted
from nasopharyngeal swabs of patient S1. RNA was subjected to one-tube
RT-PCR and amplicons were subjected to double-dilutions (#1 the most
concentrated, #9 the most diluted and #10 no amplicon). SYBR green and ROX
dyes were added and melt curve was performed. Data show derivative reporter.
These findings are consistent with the no-shift finding shown in Figure 1E.
Funding
This study was supported by a grant from the St. Michael’s Hospital Foundation (OR, AK) and a grant from CIHR # 400642 (OR).