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Supplementary Figure S3: Improved SARS-COV-2 PCR detection and genotyping with double-bubble primers

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posted on 14.09.2021, 09:10 by Menachem Ailenberg, Andras Kapus, Ori D Rotstein

Melt curve plot of double dilutions of PCR amplicons of SARS-COV-2 viral RNA extracted from nasopharyngeal swabs of patient S1. RNA was subjected to one-tube RT-PCR and amplicons were subjected to double-dilutions (#1 the most concentrated, #9 the most diluted and #10 no amplicon). SYBR green and ROX dyes were added and melt curve was performed. Data show derivative reporter. These findings are consistent with the no-shift finding shown in Figure 1E.

Funding

This study was supported by a grant from the St. Michael’s Hospital Foundation (OR, AK) and a grant from CIHR # 400642 (OR).

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