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Supplementary Figure S2. Quantification of magnetosome signals and plasmid stability in MSR-1

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posted on 11.12.2019 by Tomo Kondo, Shigehiko Yumura

Supplementary Figure S2. Quantification of magnetosome signals and plasmid stability in MSR-1. (A) Example of image processing to detect signals from magnetotactic bacteria. Briefly, cell perimeters were detected from a phase-contrast image (a). The detected boundaries were overlaid on the fluorescent image (b). Finally, the signals were detected only inside the cell (c). (B) Quantitative analysis of cells cultured for 25 days in the presence or absence of ampicillin. Cells were passaged every 2–3 days. Each number (#1–4) represents a cell line. Fluorescence (+) indicates cells with HaloTag ligand signals. n ≥ 146. (C) Image of an 1% agarose gel after electrophoresis of purified plasmids. E. coli indicates the lane with plasmids purified from E. coli cells. Each number (#1–4) represents a cell line of magnetotactic bacteria, which are the same ones shown in (B). All plasmids were linearized by a restriction enzyme reaction using MluI. The arrow indicates the predicted length of DNA bands. The gel edge was trimmed by image processing but no band enhancement was performed.

Funding

Tomo Kondo was supported by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. This research was supported by the Japan Society for the Promotion of Science [KAKENHI Grant Number 16J08310 and 19K15809 to TK]. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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