Supplementary Figure S2.pdf (102.69 kB)
Download file

Supplementary Figure S2: Improved SARS-COV-2 PCR detection and genotyping with double-bubble primers

Download (102.69 kB)
figure
posted on 14.09.2021, 09:10 by Menachem Ailenberg, Andras Kapus, Ori D Rotstein

Schematic representation of PCR primers’ locations on SARS-COV-2 genes “N” (Nucleoprotein) and “S” (Spike). Two TaqMan probes were designed for two regions on the SARS-COV-2 “N” gene: for region 1 VIC-TqM probe #12, and for region 2 FAM-TqM probe #13. For region 1, primers #5- #8 flank primers #5 and #6 and therefore produce a longer amplicon than the later primers, but both sets can utilize VIC-TqM probe #12. For region 2, primers #9- #10 utilize FAM-TqM probe #13. Primer mixes #3, #5, #7, #9 are random-coil normal type. Primer mixes #4, #6, #8, #10 are D-B type. Region 3 represents the spike gene and primer mixes 11a+11c and 11b+11C were designed to preferentially amplify targets of wild type or N501Y mutation targets, respectively. For specific Sequences of the primers seen Table S1.

Funding

This study was supported by a grant from the St. Michael’s Hospital Foundation (OR, AK) and a grant from CIHR # 400642 (OR).

History