Supplementary Figure S1: Comparative in situ hybridization protocols in zebrafish
Protocol for: Comparative in situ hybridization protocols in zebrafish
Supplementary Figure 1. Probes were analyzed for quality on agarose gels and dot blot. A. Cabin1 antisense and sense probe dilutions (straight probe, 1; 1:5 dilution, and 1:10 dilution) show clear, discrete bands on a non-denaturing agarose gel. B. Fluorescein (FLU)- and digoxigenin (DIG)-labeled Cabin1 probe dilutions were spotted in nylon membranes for dot blot analysis.