Supplementary Figure S1: Comparative in situ hybridization protocols in zebrafish

Protocol for: Comparative   in situ hybridization protocols in zebrafish

Supplementary   Figure 1. Probes were analyzed for quality on agarose gels and dot blot. A. Cabin1 antisense and   sense probe dilutions (straight probe, 1; 1:5 dilution, and 1:10 dilution)   show clear, discrete bands on a non-denaturing agarose gel. B. Fluorescein (FLU)- and digoxigenin   (DIG)-labeled Cabin1 probe dilutions were spotted in nylon membranes for dot   blot analysis.