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Supplementary Figure – Comprehensive analysis of epigenetic modifications and immune-cell infiltration in tissues from SLE patients

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posted on 16.12.2021, 10:41 by Figshare Future Science GroupFigshare Future Science Group, Zhenghao He, Shihang Zhou, Ming Yang, Zhidan Zhao, Yang Mei, Yue Xin, Ming Zhao, Haijing Wu, Qianjin Lu

Supplementary Figure 1

Identification of DEGs and enrichment of DEGs

(A)Volcano plot of datasets in normal and SLE samples from GSE20864. Blue plots represent expressions of genes with P < 0.05 and log2FC < −0.8. Red plots represent expressions of genes mRNA with P < 0.05 and log2FC > 0.8. Grey plots represent genes expressed mRNA normally. The X-axis means the log2 of fold change in expressions of genes between primary and metastatic samples. The Y-axis means the −log10 of the P value of each gene. (B) Volcano plot of datasets in normal and SLE samples from GSE112087. (C) Volcano plot of datasets in normal and SLE samples from GSE122459. (D) Volcano plot of datasets in normal and SLE samples from GSE144390. (E) Enrichment of DEGs in 4 datasets.

Supplementary Figure 2

Correlation analysis of immune cells and key genes

The red circle represents positive correlation, and the purple circle represents negative correlation (P<0.05).

Supplementary Figure 3

Identification of different immune cells in single cell analysis

(A) PBMC cells from 5 normal subjects and 7 SLE patients are divided into 19 clusters. Each point represents a single cell, colored according to clusters. (B) The amount of marker genes expressed by each cluster. (C) Relative frequency of expression of each immune cell in SLE and HC

Supplementary Figure 4

Epigenetic analysis of SLE

(A) Enrichment of hypomethylation gene. (B) Volcano plot of datasets in normal and SLE samples from GSE37426. (C) Heatmap of DEmiRNAs between normal and SLE samples from GSE37426. (D) Volcano plot of datasets in normal and SLE samples from GSE102547. (E) Heatmap of DElncRNAs between normal and SLE samples from GSE102547. (F) Volcano plot of datasets in normal and SLE samples from GSE84655. (G) Heatmap of DEcircRNAs between normal and SLE samples from GSE84655.

Supplementary Figure 5

The mRNA expression of 5 key genes in HC and autoimmune diseases

The relative mRNA expression of RSAD2, OAS2, IFIT1, IFIT3, PLSCR1 in the PBMCs of HC, SLE, RA, DM, SS.

Supplementary Figure 6

The protein expression of IFIT1 and RSAD2 in SLE and HC skin lesions.

(A) The statistical analysis percentage of IFIT1 positive cells between SLE and HC. (B) The statistical analysis percentage of RSAD2 positive cells between SLE and HC.

Funding

National Natural Science Foundation of China (No.81972943,No.81830097)

Hunan Talent Young Investigator (No. 2019RS2012)

Hunan Outstanding Young Investigator (No. 2020JJ2055)

Hunan Urgency Project (2020SK3005)

CAMS Innovation Fund for Medical Sciences (CIFMS) (2019-I2M-5-033)

History