Supplementary Figure – Comprehensive analysis of epigenetic modifications and immune-cell infiltration in tissues from SLE patients
Supplementary Figure 1
Identification of DEGs and enrichment of DEGs
(A)Volcano plot of datasets in normal and SLE samples from GSE20864. Blue plots represent expressions of genes with P < 0.05 and log2FC < −0.8. Red plots represent expressions of genes mRNA with P < 0.05 and log2FC > 0.8. Grey plots represent genes expressed mRNA normally. The X-axis means the log2 of fold change in expressions of genes between primary and metastatic samples. The Y-axis means the −log10 of the P value of each gene. (B) Volcano plot of datasets in normal and SLE samples from GSE112087. (C) Volcano plot of datasets in normal and SLE samples from GSE122459. (D) Volcano plot of datasets in normal and SLE samples from GSE144390. (E) Enrichment of DEGs in 4 datasets.
Supplementary Figure 2
Correlation analysis of immune cells and key genes
The red circle represents positive correlation, and the purple circle represents negative correlation (P<0.05).
Supplementary Figure 3
Identification of different immune cells in single cell analysis
(A) PBMC cells from 5 normal subjects and 7 SLE patients are divided into 19 clusters. Each point represents a single cell, colored according to clusters. (B) The amount of marker genes expressed by each cluster. (C) Relative frequency of expression of each immune cell in SLE and HC
Supplementary Figure 4
Epigenetic analysis of SLE