Supplementary Figure 3. Silver nanoparticles induce apoptosis via NOX4-derived mitochondrial ROS and ER stress in colorectal cancer cells
Supplementary Figure 3. AgNPs induced PARP cleavage through mitochondrial ROS in HCT116 cells. HCT116 cells were pretreated with or without 0.5 μM DPI for 4 h and incubated with 5 μg/ml AgNPs for 24 h. Representative images obtained by confocal fluorescence microscopy stained with cleaved PARP antibody. Cleaved PARP-positive cells were observed following the AgNP treatment while cleaved PARP-positive cells were apparently decreased in cells pretreated with DPI.