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Supplementary Figure 2. Silver nanoparticles induce apoptosis via NOX4-derived mitochondrial ROS and ER stress in colorectal cancer cells

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posted on 20.05.2021, 09:27 by Juan-Hua Quan, Fei Fei Gao, Jia-Qi Chu, Guang-Ho Cha, Jae-Min Yuk, Weiyun Wu, Young-Ha Lee

Supplementary Figure 2. AgNPs up-regulated NOX4 mRNA levels in HCT116 cells dose dependently. NOX isoforms were detected by PCR using specific primers for NOX isoforms. GAPDH was used as a loading control. NOX4 mRNA was significantly increased by the treatment of AgNPs in a dose-dependent manner but NOX1, NOX2 and NOX3 expression was not detected in HCT116 cells. In addition, 0.2 μg/mL AgNPs significantly increased NOX5 mRNA levels, and 1-25 μg/mL AgNPs gradually down-regulated NOX5 expression dose dependently.


This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2019R1A2C1088346) at Chungnam National University, and the National Natural Science Foundation of China (81971389 and 81771612), the Characteristic Innovation Projects of Guangdong Universities (2018KTSCX079, 2018KTSCX081) and the Guangdong Basic and Applied Basic Research Foundation (2019A1515011888 and 2019A1515011715).