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Supplementary Figure 2: Robust co-immunoprecipitation with mass spectrometry for C. elegans by using solid-phase enhanced sample preparation

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posted on 17.03.2022, 13:59 by Gülkiz Baytek, Oliver Popp, Philipp Mertins, Baris Tursun
Supplemental figure 2: Correlation of COlP-MS replicate experiments DNAse versus benzonase. Correlation matrices of anti-HA ColP-MS to precipitate HA-tagged HMG-3 or HMG-4 plotted as a heatmap, showing the Pearson correlations between control samples and ColP-MS experiments with either DNAse or benzonase treatments. Replicate samples of treated Colp experiments show overall high correlation for all detected proteins prior to calculating the significantly enriched proteins.

Funding

Thanks to Sergej Herzog for technical assistance and the CGC, funded by NIH P40OD010440, for providing strains. This work was partly sponsored by the ERC-StG-2014-637530 and the Max Delbrueck Center for Molecular Medicine in the Helmholtz Association. All procedures conducted in this study were approved by the Berlin State Department for Health and Social (LaGeSo). The authors declare that they have no competing interests. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

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