Supplementary Figure 2: Reduced-serum methods for contact-based co-culture of human dermal fibroblasts and epidermal keratinocytes
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Suppl Figure 1. Morphology and proliferation of HDFa and HaCaT, mono-culture and co-culture, under different media conditions. (A) HDFa growing in Fibroblast Growth Medium (FGM; containing 2% FBS and growth factors) with characteristic spindle-shaped morphology and HaCaT (immortalized) growing in Keratinocyte Growth Medium (KGM; serum-free containing growth factors) with typical mosaic, cobblestone morphology. (B) Co-seeding of HDFa and HaCaT (in a 1:1 ratio) in MEM alone (with no growth supplements or FBS; a-d) and MEM with added 10% FBS (no growth supplements) (e-h). In the absence of serum and growth supplements (a-d), HDFa show poor proliferation, and HaCaT fail to attach and proliferate. In the presence of 10% FBS (no growth supplements, e-h), HDFa and HaCaT shows minimal proliferation, but HDFa do not form typical sheaths around HaCaT colonies (Day 3 onwards). Scale bars = 150 µm.