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Supplementary Figure 2: Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification

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posted on 07.04.2021, 13:19 by Bàrbara Reynés, Francisca Serra, Andreu Palou

Supplementary Figure 2. A. Visual detection at 20, 25, and 30 minutes of LAMP reaction with 5·103 copies of synthetic SARS-CoV-2 RNA (3 replicates) or Non-template control (NTC) (1 replicate), for each set of N and ORF1ab primers, at 63ºC or 65ºC. B. Visual detection at 15, 20, and 25 minutes of LAMP reaction at 65ºC with 5·103 copies of synthetic SARS-CoV-2 RNA (3 replicates) or Non-template control (NTC) (1 replicate), with 1 or 1.5µL for each set of N and ORF1ab primers or their combination (1µL of N+1µL of ORF1ab, or 1.5µL of N+1.5µL of ORF1ab). The assay was conducted in 8-wells PCR strips and the image has been taken from the front of the strip.

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Universitat de les Illes Balears and Alimentómica SL

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