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Supplementary Figure 2 - Small extracellular vesicles from iPSC-derived mesenchymal stem cells ameliorate tendinopathy pain by inhibiting mast cell activation

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posted on 15.03.2022, 09:47 by Renzhi Gao, Teng Ye, Zhaochen Zhu, Qing Li, Juntao Zhang, Ji Yuan, Bizeng Zhao, Zongping Xie, Yang Wang

Supplementary Information

Abstract

Aims: This study aimed to explore the effect of iMSC-sEVs on acute pain and investigate the underlying mechanisms.

Materials & Methods: The pathology of tendon was accessed by H&E staining, immunohistochemical and immunofluorescent staining. The pain degree was measured by pain-related behaviors. In vitro, we performed -Hexosaminidase release assay, RT-qPCR, Toluidine blue staining, ELISA and RNA-seq.

Results: iMSC-sEVs effectively alleviated acute pain in tendinopathy as well as inhibited activated mast cell infiltration and interactions with nerve fibers in vivo. In vitro, iMSC-sEVs reduced the degranulation of mast cells, the expression of proinflammatory cytokines and genes involved in the HIF-1 signaling pathway.

Conclusions: This study demonstrated iMSC-sEVs relieved tendinopathy-related pain through inhibiting mast cells activation via the HIF-1 signaling pathway.

Funding

National Natural Science Foundation of China (81870972, 82072550).

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