Supplementary Figure 2 - Low expression of Trefoil factor 3 in papillary thyroid carcinoma may correlate with poor prognoses but high immune cell infiltration
figureposted on 16.11.2021, 14:12 by Lei Yang, Xiwei Zhang, Jiyin Zhang, Yuwei Liu, Tingting Ji, Jianing Mou, Xiaolian Fang, Xin Zhang, Jun Chen
Supplementary Figure 2: Low expression of Trefoil factor 3 in papillary thyroid carcinoma may correlate with poor prognoses but high immune cell infiltration
The heatmap of expression profile for six genes in 254 samples of SRA database
Background: Papillary thyroid carcinoma (PTC) is most common endocrine malignancies with favorable prognosis. However, current treatments and prognostic markers have not been clearly identified.
Methods: Gene expression data from primary PTC were downloaded from the GEO database and subjected to two analyses of differentially expressed genes (DEGs), followed by intersecting individual and integrated DEGs analyses as well as gene set enrichment analysis. SRA, TCGA database and IHC, qRT-PCR of TFF3 were performed to validate the results. Finally, the relationship between gene expression and disease-free survival as well as immune cell infiltration were investigated.
Results: Six critical DEGs and several tumor-enriched signaling pathways were identified. IHC and qRT-PCR validated the low expression of TFF3 in PTC. TFF3 and FCGBP are coexpressed in PTC, and patients with lower gene expression had worse disease-free survival but higher immune cell infiltration.
Conclusion: TFF3 was significantly underexpressed and may function with FCGBP synergistically in PTC.