Background: This study aimed to investigate the expression of regeneration-related
genes in canine urine during bladder repair.
Materials & methods:
Canine urine samples were collected after partial cystectomy. Regenerative mRNA
of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF),
key stem cell transcription factors, and cholinergic signals were detected.
Results:
HIF-1α, VEGF, CD44, interleukin-6, and prominin 1 expression in canine urine
after partial cystectomy exhibited two similar peaks at ~2 weeks. HIF-1α and
VEGF expression was higher in the afternoon than in the morning. The expression
of key stem cell transcription factors and cholinergic signals also exhibited a
rhythm along with bladder healing.
Conclusions: The expression of
HIF-1α, VEGF, key stem cell transcription factors, and cholinergic signals
exhibited a time curve distribution during canine bladder healing. The
expression trend of some regenerative genes was similar during bladder healing,
and a cooperative effect may exist.
