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Supplementary Figure 1: Strand specific detection of overlapping transcripts via purification involving denaturation of biotinylated cDNA

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posted on 2020-05-06, 09:23 authored by Faizan Uddin, Madhulika Srivastava

Supplementary Figure 1. Enrichment of biotinylated cDNA using streptavidin magnetic beads. Antisense RNA was generated by in vitro transcription of a plasmid clone having Eb region as the insert. 10ng of RNA was reverse transcribed using biotinylated m1397 as primer. The resultant cDNA was subjected to streptavidin magnetic beads based purification. Abundance of cDNA before and after purification were estimated by qPCR (m1397-m1398r). Relative levels of enriched biotinylated cDNA normalized to total cDNA before enrichment in three cDNA samples are shown. Error bars represent SEM (n=3). Primer locations as in Figure 4.

Supplementary Table 1. Sequences of the primers used in RT-PCR experiments


The study was supported by a grant [EMR/2016/006230] received from SERB, Dept. of Science and Technology, Govt of India.