Supplementary Figure 1: Staining kinetics of PI and Hoechst in 3D organoids.
figureposted on 20.06.2019, 09:48 by Stefanie Mueller, Konstantin J. Bode, Matthias Schweinlin, Marco Metzger, Thomas Brunner
Intact (A) and damaged (B) organoids were stained with PI&Hoechst. Subsequently, brightfield (BF) and fluorescence microscopic images were taken 5, 10 ,15 ,30, and 60 minutes after the dyes were added to culturing medium. (scale bar = 100 µm)