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Supplementary Figure 1. Silver nanoparticles induce apoptosis via NOX4-derived mitochondrial ROS and ER stress in colorectal cancer cells

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posted on 2021-05-20, 09:27 authored by Juan-Hua Quan, Fei Fei Gao, Jia-Qi Chu, Guang-Ho Cha, Jae-Min Yuk, Weiyun Wu, Young-Ha Lee

Supplementary Figure 1. AgNPs induced morphological changes in HCT116 cells dose- and time-dependently. HCT116 cells were treated with various concentrations of AgNPs for 24 h or 5 μg/mL AgNPs for the indicated times. (A, B) HCT116 cells were fixed and probed against α-tubulin (red). The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Untreated control HCT116 cells displayed a well-developed array of hair-like microtubule networks of slim fibrous microtubules wrapped around the cell nucleus. In contrast, cells treated with AgNPs showed a tubulin staining pattern that was diffuse and disorganized.


This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2019R1A2C1088346) at Chungnam National University, and the National Natural Science Foundation of China (81971389 and 81771612), the Characteristic Innovation Projects of Guangdong Universities (2018KTSCX079, 2018KTSCX081) and the Guangdong Basic and Applied Basic Research Foundation (2019A1515011888 and 2019A1515011715).