Supplementary Figure 1. Silver nanoparticles induce apoptosis via NOX4-derived mitochondrial ROS and ER stress in colorectal cancer cells

Supplementary Figure 1. AgNPs induced morphological changes in HCT116 cells dose- and time-dependently. HCT116 cells were treated with various concentrations of AgNPs for 24 h or 5 μg/mL AgNPs for the indicated times. (A, B) HCT116 cells were fixed and probed against α-tubulin (red). The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Untreated control HCT116 cells displayed a well-developed array of hair-like microtubule networks of slim fibrous microtubules wrapped around the cell nucleus. In contrast, cells treated with AgNPs showed a tubulin staining pattern that was diffuse and disorganized.