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Supplementary Figure 1: Robust co-immunoprecipitation with mass spectrometry for C. elegans by using solid-phase enhanced sample preparation

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posted on 17.03.2022, 13:59 by Gülkiz Baytek, Oliver Popp, Philipp Mertins, Baris Tursun

Supplemental Figure 1: Correlation of ColP-MS replicate experiments. (A) Correlation matrix of anti-HMG-3/4 ColP-MS without and with SP3 plotted as a heatmap, showing the Pearson correlations between control samples (unspec. IgG) or anti-HMG-3/4 ColP-MS. Replicate samples of respective experiments show low correlation without SP3 clean-up. (B) Data from the parallel study. “(Bayket et al., 2021 bioRxiv, doi.org/10.1101/2021.02.14.431134, and co-submitted to BioTechniques)./ Correlation matrix plotted as a heatmap, showing the Pearson correlations between 11 Co-IP and 10 control samples of anti-HA ColP-MS with SP3 clean-up to determine MRG-1 protein interactions. Wildtype (N2) with no HA tag was used as a negative control to eliminate background and mrg-1 :: 3xHA (CRISPR/Cas9 generated) strain was used to identify MRG-1 interacting proteins.

Funding

Thanks to Sergej Herzog for technical assistance and the CGC, funded by NIH P40OD010440, for providing strains. This work was partly sponsored by the ERC-StG-2014-637530 and the Max Delbrueck Center for Molecular Medicine in the Helmholtz Association. All procedures conducted in this study were approved by the Berlin State Department for Health and Social (LaGeSo). The authors declare that they have no competing interests. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

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