Supplementary Figure 1: Robust co-immunoprecipitation with mass spectrometry for C. elegans by using solid-phase enhanced sample preparation
Supplemental Figure 1: Correlation of ColP-MS replicate experiments. (A) Correlation matrix of anti-HMG-3/4 ColP-MS without and with SP3 plotted as a heatmap, showing the Pearson correlations between control samples (unspec. IgG) or anti-HMG-3/4 ColP-MS. Replicate samples of respective experiments show low correlation without SP3 clean-up. (B) Data from the parallel study. “(Bayket et al., 2021 bioRxiv, doi.org/10.1101/2021.02.14.431134, and co-submitted to BioTechniques)./ Correlation matrix plotted as a heatmap, showing the Pearson correlations between 11 Co-IP and 10 control samples of anti-HA ColP-MS with SP3 clean-up to determine MRG-1 protein interactions. Wildtype (N2) with no HA tag was used as a negative control to eliminate background and mrg-1 :: 3xHA (CRISPR/Cas9 generated) strain was used to identify MRG-1 interacting proteins.