Supplementary Figure 1: Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification

Supplementary Figure 1. A. Visual detection at 16 and 20 minutes of LAMP reaction with 5·10³ copies of synthetic SARS-CoV-2 RNA (3 replicates) or Non-template control (NTC) (1 replicate) at 65ºC. LAMP reaction was performed with 0.87µM glycerol (Gly), 40mM Guanidine hydrochloride (GHC), without any extra compound (None) or with different concentration of MgCl₂ (0.2 and 1.0mM) and dNTPs (0.5, 1.0 and 1.5mM). B. Visual detection at 20 minutes of LAMP reaction with 5·10³ copies of synthetic SARS-CoV-2 RNA (5 replicates) or Non-template control (NTC) (2 replicates) at 65ºC. LAMP reaction was performed with 0.24µM Bovine Serum Albumin (BSA), 40mM Guanidine hydrochloride (GHC), with both BSA (0.24µM) + GHC (40mM) or without any extra compound (None). The assay was conducted in 96-wells PCR plates and the image has been taken from the bottom.