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Supplementary Figure 1: Effect of mismatch between types of viral nucleic acid and intended targets of extraction kits on PCR-based testing

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posted on 2022-06-28, 07:58 authored by Jin-Ho Kim, Hanjun Kim, Kyoung-Jin Yoon

Supplementary Figure 1. Efficiency of DNA, RNA, and total nucleic acid (TNA) extraction kits in isolating DNA and/or RNA from serially diluted DNA viruses [bovine herpesvirus type 1 (BHV-1) and porcine circovirus type 2 (PCV-2)] and RNA viruses [porcine epidemic diarrhea virus (PEDV) and porcine rotavirus type A (PoRVA)] as measured by conventional polymerase chain reaction (PCR), nested (n) PCR, reverse transcription (RT)-PCR, and/or RT-nPCR assays. The expected molecular size of the correct PCR product from BHV-1, PEDV, PCV-2, and PoRVA is 235, 251, 966, and 121 bp, respectively. PC and NC stand for positive control and negative control, respectively. The thick line on the top of each digitalized gel picture indicates serial dilutions of each virus produced the positive result by the given extract kit and the given PCR.

Funding

The study was supported in part by ISU VDL’s internal fund for assay R&D. Dr. Jin-Ho Kim was supported by the National Research Foundation of Korea (NRF-2018K1A4A3A01064257). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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