Supplementary Figure 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach
figureposted on 2019-03-21, 09:57 authored by Yuko Takagi, Hirokazu Nosato, Motomichi Doi, Koji Furukawa, Hidenori Sakanashi
T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox was used to count the same sample as(A).The same still images used in (B)were analyzed.
Development of a genetic alteration method that contributes to discovery of Chagas' disease drug discovery target
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