Supplemental Figure 1.pdf (524.88 kB)

Supplemental Figure 1.pdf

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posted on 2022-02-14, 08:53 authored by Lucy M. Kimbley, Rachel Parker, Jack Harrington, Robert C Walker, Ben L Grace, Jonathan WestJonathan West, Timothy J Underwood, Matthew J.J. Rose-Zerilli

Supplemental Figure 1: Pre-combined analysis. (A) UMAP clustering of nuclei isolated by the EZ method with cell types identified. (B) UMI count of EZ nuclei across clusters. (C) Gene count of EZ nuclei stratified by cell type cluster. (D) UMAP clustering of nuclei isolated by the citric acid method with cell types identified. (E) UMI count of citric acid nuclei across clusters. (F) Gene count of citric acid nuclei stratified by cell type cluster.

Funding

T.J.U is supported by a Royal College of Surgeons of England and Cancer Research UK Advanced Clinician Scientist Fellowship (A23924). R.C.W is supported by a Cancer Research UK Clinical Research Training Fellowship (A25162). Development of Drop-seq technology in Southampton was funded by MRC grant (MC_PC_15078). This work was supported by a grant from the Chan Zuckerberg Initiative (Human Cell Atlas Pilot project: START: Standardization of Single-Cell and Single-Nucleus RNA-Seq Protocols for Tumors) to J.J.W, T.J.U. and M.J.J.R-Z. and a grant from the Southampton CR UK Development Fund to J.J.W, T.J.U. and M.J.J.R-Z.

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Keywords

nuclei RNA Sequencing tissue sampling esophagus transcriptome single cell Medical Genetics (excl. Cancer Genetics)Otorhinolaryngology

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