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Safety and efficacy of mutant neoantigen-specific T cells treatment combined anti-PD-1 therapy in stage IV solid tumors. supplementary figures

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posted on 24.03.2022, 08:48 authored by Shunchang Jiao, Xiudi Chen, Xin Yi, Xuefeng Xia, Yanfang Guan, Qi Xiong, Quan Gan, Xiao Zhao, Guoqing Zhang, Shengjie Sun, XiaoLing Zhang, Dan Wang, Pansong Li, Liyan Ji, Boyu Qin, Tianfu Zhang, Zishan Zhou, Wei Sheng, Bo Yang, Qi Song, Wei Guo

Figure S1. IFN-γ positive percent of CD8+ T cells in PBMCs and antigen specific CIK cells. (A) (B) IFN-γ positive percent in CD3+CD8+ T cells in the uncultured PBMC; (C) (D) IFN-γ positive percent in CD3+CD8+ T cells antigen-specific CIK cells in patients after culture. 1# MTAP/SPINK1 Merged Gene peptide: VLLPRHMKV; 2# FERS70F: NYVSNVSKF; 3# SPINK1: FLLSALALL; 4# CLCN6F507S: ALIGAAASL; 5# MTAP: AESFMFRTW; 6# TAP1V252A: RLSLFLALV; 7# MAN2C1Q255R: FLQGRNFFL; 8# CLCN6-wt: ALIGAAAFL; 9# TAP1-wt: RLSLFLVLV; 10# MAN2C1-wt: FLQGQNFFL.

Figure S2. CD137 and IFN-γ coexpress in the neoantigen-activated CD3+CD8+ T cells, and sort CD137 positive CD8+ T cells by Flow Cytometry (A) Percentage of CD137 and IFN-γ co-expressing cells in antigen-specific CIK cells stimulated by tumor antigen 7# MAN2C1 Q255R: FLQGRNFFL. The antigen-specific CIK cells were cultured for about 10 days, and then 50 ug/mL tumor antigen 7# MAN2C1 Q255R: FLQGRNFFL was added to stimulate the antigen-specific CIK cells for 12 h, after which flow, cytometry was used to measure the percentage of CD137 and IFN-γ co-expressing cells in CD3+CD8+ T cells. MOCK referred to antigen-specific CIK cells not stimulated by 7# MAN2C1 Q255R. 7# MAN2C1 Q255R referred to the antigen-specific CIK cells stimulated by 7# MAN2C1 Q255R. (B) CD3+CD8+CD137+ T cells activated by the stimulation of 7# MAN2C1 Q255R in tumor antigen specific CIK cells sorted by flow cytometry. The percentage of CD3+CD8+CD137+ T cells was about 0.6% before the sorting, which was concentrated by about 30 folds to 18.3% after the sorting.

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