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Real-time in situ monitoring of CRM-197 and polysaccharide conjugation reaction by fluorescence spectroscopy: supplementary figures

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posted on 2023-05-04, 18:52 authored by Anumita Saha-Shah, Joseph Smith, Janelle Konietzko, Emily Costell, Patrick M McHugh, Justin P Lo-Mont, Nicole M Ralbovsky, Louis Obando, Sheng-ching Wang, Emmanuel Appiah-Amponsah, Ian Mangion

Aims: Process analytical technology (PAT) is increasingly being adopted within the pharmaceutical industry

to build quality into a process. Development of PAT that provides real-time in situ analysis of critical quality

attributes are highly desirable for rapid, improved process development. Conjugation of CRM-197 with

pneumococcal polysaccharides to produce a desired pneumococcal conjugate vaccine is a significantly

intricate process that can tremendously benefit from real-time process monitoring. Methods: In this work,

a fluorescence-based PAT methodology is described to elucidate CRM-197-polysacharide conjugation

kinetics in real time. Results & conclusion: In this work, a fluorescence-based PAT methodology is described

to elucidate CRM-197-polysacharide conjugation kinetics in real time.

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