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Protease-targeting peptide-functionalized porous silicon nanoparticles for cancer fluorescence imaging Supplementary figure

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posted on 2022-11-16, 10:44 authored by Jayasree S Kanathasan, Uma Devi Palanisamy, Ammu K Radhakrishnan, Srikumar Chakravarthi, Tan Boon Thong, Varghese Swamy

Porous silicon (pSi) nanoparticles (NPs) functionalized with suitable targeting ligands are

now established cancer bioimaging agents and drug-delivery platforms.With growing interest in peptides

as tumor-targeting ligands, much work has focused on the use of various peptides in combination

with pSi NPs for cancer theranostics. Here, the authors investigated the targeting potential of pSi NPs

functionalized with two types of peptide, a linear 10-mer peptide and its branched (Y-shaped) equivalent,

that respond to legumain activity in tumor cells. Results: In vitro experiments established that the

linear peptide-pSi NP conjugate had better aqueous stability under tumor conditions and higher binding

efficiency (p < 0.001) toward legumain-expressing cells such as RAW 264.7 cells compared with that of

its branched equivalent. In vivo studies (analyzed using ex vivo fluorescence) with the linear peptide-pSi

NP formulation using a syngeneic mouse model of breast cancer showed a higher accumulation (p > 0.05)

of linear peptide-conjugated pSi NPs in the tumor site within 4 h compared with nonconjugated pSi

NPs. These results suggest that the linear peptide-pSi NP formulation is a nontoxic, stable and efficient

fluorescence bioimaging agent and potential drug-delivery platform.


Ministry of Higher Education (Malaysia), (Grant / Award Number: 'Fundamental Research Grant Scheme grant FRGS/1/201')


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