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NNM-2021-0038: Supplementary Figure 5. (A) Agarose gel electrophoresis patterns of the vector/pCRISPR complexes in different weight ratios. (B) Results of the agarose gel retardation assay after treating the complexes with negatively charged dextran polymer (top) and 200-mM aqueous NaCl (bottom). (C) Intensity and quantity of pCRISPR using image J software, determined by arranging pieces of pCRISPR bonds obtained from gel retardation data (A). (D) Analysis of the data obtained from the Image J s

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posted on 2021-07-22, 11:15 authored by Akbar Hasanzadeh, Fatemeh Radmanesh, Elaheh Sadat Hosseini, Iman Hashemzadeh, Jafar Kiani, Marzieh Naseri, Helena Nourizadeh, Yousef Fatahi, Behjat Kheiri Yeghaneh Azar, Behnaz Golnari Marani, Ali Beyzavi, Vahid Pirhajati Mahabadi, Mahdi Karimi

Supplementary Figure 5. (A) Agarose gel electrophoresis patterns of the vector/pCRISPR complexes in different weight ratios. (B) Results of the agarose gel retardation assay after treating the complexes with negatively charged dextran polymer (top) and 200-mM aqueous NaCl (bottom). (C) Intensity and quantity of pCRISPR using image J software, determined by arranging pieces of pCRISPR bonds obtained from gel retardation data (A). (D) Analysis of the data obtained from the Image J software. (L and P stand for ladder and pCRISPR, respectively).

Funding

Funding support was received from the Iran University of Medical Science (Grant 97-3-75-13318). The authors also acknowledge the Iran Science Elites Federation for funding this work.

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    Nanomedicine

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    carbon dotsnanovectorCRISPR/Cas9gene deliveryvitamin D3Medical Biochemistry: Nucleic Acids

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