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Loop-mediated isothermal amplification assay in combination with lateral flow dipstick in HSV type 1: Supplementary figures

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posted on 2023-02-03, 12:41 authored by Fei Gao, Zhuoshi Wang, Mingqi Zhang, Yan Zhao, Tao Yao, Ling Xu, Xiangdong He, Bing Wang, Wei He

Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV-

1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral

flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime

PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP

reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to 10 copies/μl and

no cross-reactivity with other pathogens was observed. Moreover, LAMP-LFD had similar positive rates

to qPCR in clinical samples, and sensitivity and specificity were highly consistent with those of qPCR.

Conclusion: LAMP-LFD assay is a rapid technique for the diagnosis of HSV-1.

Funding

This work was supported by the Shenyang Science and Technology Project (no. 20-301-4-00) and Natural Science Funding of Liaoning Province (nos. 2019-ZD-0994 and 2020-BS-292).

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    Future Virology

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    Keywords

    HSV type 1herpetic keratitislateral flow dipstickloop-mediated isothermal amplificationquantitative PCR

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