Loop-mediated isothermal amplification assay in combination with lateral flow dipstick in HSV type 1: Supplementary figures
Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV-
1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral
flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime
PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP
reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to 10 copies/μl and
no cross-reactivity with other pathogens was observed. Moreover, LAMP-LFD had similar positive rates
to qPCR in clinical samples, and sensitivity and specificity were highly consistent with those of qPCR.
Conclusion: LAMP-LFD assay is a rapid technique for the diagnosis of HSV-1.